Sure seems like it would, because your creating head pressure on the line, the deeper the line the more the reverse effect you will get from that head pressure, which would be pushing back on any beneficial vacuum created thereby reducing vacuum.
Do you have a gauge at the top of the line. Try putting the line in the bottom of the tank. Check your gauge, then have your line drip into the top of the tank and check your gauge again. It may not be detectable on the gauge however... not sure.
I sense that doing this with a drop into the bottom of a bucket will cause problems, both in hydro dynamics and sanitation.
Sanitation for certain a problem here.
Higher then atmospheric pressure is pushed back towards any column of sap generating vacuum or the tap hole itself if no column/vacuum exists (i.e. drops to buckets )
If you think it's easy to make good money in maple syrup .... then your obviously good at stealing somebody's Maple Syrup.
Favorite Tree: Sugar Maple
Most Hated Animal: Sap Sucker
Most Loved Animal: Devon Rex Cat
Favorite Kingpin: Bruce Bascom
40 Sugar Maple Taps ... 23 in CT and 17 in NY .... 29 on gravity tubing and 11 on 5G buckets ... 2019 Totals 508 gallons of sap, 7 boils, 11.4 gallons of syrup.
1 Girlfriend that gives away all my syrup to her friends.