15- 20 % alcohol/water solution pumped into the pulled tap-dropline after making a single or double knot in dropline. the knot should hang below line of sight on lateral. The old spile is snipped off and empty line capped (variety of ways) A portion of the Alcohol will lay in the bottom of knots and acts in same way as a peetrap in a waste water system. the vapours are now locked in dropline till tapping season as long as cap is not removed (ie windfalls or wildlife). I have had samples tested for bacteria ( both knotted-with and without end caps. Capped ones very low in bacteria but soon as air hits (uncapped ) gave high readings.OMAFRA (Ontario) did the samples.There is controversy over the proper % solution- some are using 70 % to 100% alcohol at considerable cost.